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| Registros recuperados : 19 | |
| 5. |  | FERREIRA, T. M. M.; SILVA, V. N. B.; SOUZA JUNIOR, M. T.; SOUSA, C. A. F. de. Análise morfofisiológica de Portulaca olerecea L. submetidos a estresse salino. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 18.| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | FERREIRA, T. M. M.; FERREIRA FILHO, J. A.; LEAO, A. P.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Structural and functional analysis of stress-resonsive genes and their selected promoters from young oil palm (ELAEIS GUINEENSIS) under two abiotic stresses. In: BRAZILIAN CONGRESS OF GENETICS, 67., 2022, Natal, RN. Porto Alegre: Sociedade Brasileira de Genética, 2022. p. 320| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | SANTOS, T. T.; CASARI, R. A. das C. N.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; SOUZA JUNIOR, M. T.; SILVA, V. N. B.; FERREIRA, T. M. M.; SOUSA, C. A. F. de. Discrimination of responses of corn genotypes to drought through physiological, growth, and yield traits. Pesquisa Agropecuária Brasileira, v. 56, e01948, 2021. Título em português: Discriminação das respostas de genótipos de milho à seca por meio de características fisiológicas e de crescimento e produção.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Agroenergia; Embrapa Meio-Norte; Embrapa Unidades Centrais. |
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| 11. | | CASARI, R. A. C. N.; SILVA, V. N. B.; FERREIRA, T. M. M.; SANTOS, T. T.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; SOUZA JUNIOR, M. T.; SOUSA, C. A. F. de. Development of a protocol aiming at the estimation of pigment contents in maize leaves by hyperspectral remote sensing. In: BRAZILIAN CONGRESS OF PLANT PHYSIOLOGY, 16., 2017, São Pedro, SP. Abstract book. [S.l.]: SBFV, 2017. p. 107-108. Na publicação: Cunha BADB, Souza Jr MT.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 12. | | CASARI, R. A. das C. N.; SOUSA, C. A. F. de; SANTOS, T. T.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; SOUZA JUNIOR, M. T.; DIAS, B. B. A.; FERREIRA, T. M. M.; SILVA, V. N. B. Development of a protocol aiming at the estimation of pigment contents in maize leaves by hyperspectral remote sensing. In: BRAZILIAN CONGRESS OF PLANT PHYSIOLOGY, 16., 2017, São Pedro, SP. Abstract - [S.l.]: Sociedade Brasileira de Fisiologia Vegetal, 2017. p. 107-108.| Biblioteca(s): Embrapa Agroenergia. |
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| 13. | | FERREIRA, T. M. M.; SALGADO, F. F.; SOUZA, O. C. A.; SILVA, R. V.; SILVA, V. N. B.; OLIVEIRA, P. A. de; ROCHA, T. L.; SOUZA JUNIOR, M. T. Genetic engineering of purslane (Portulaca oleracea L.). In: OLIVEIRA, M. S. de; ANDRADE, E. H. de A.; KUMAR, R. Medicinal plants: chemical, biochemical, and pharmacological approaches. IntechOpen, 2023| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | SILVA, V. N. B.; SILVA, T. L. C. da; FERREIRA, T. M. M.; RODRIGUES NETO, J. C.; LEAO, A. P.; RIBEIRO, J. A. de A.; ABDELNUR, P. V.; VALADARES, L. F.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Multi-omics analysis of young Portulaca Oleracea L. plants' responses to High NaCl doses reveals insights into pathways and genes responsive to salinity stress in this halophyte species. Phenomics, 2022. Published online: 15 June 2022.| Biblioteca(s): Embrapa Agroenergia; Embrapa Meio-Norte. |
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| 15. | | SILVA, V. N. B.; FERREIRA, T. M. M.; PAIVA, D. S. de; CASARI, R. A. das C. N.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; SOUZA JUNIOR, M. T.; SOUSA, C. A. F. de. Effect of drought on the gas exchange of maize genotypes with different levels of drought tolerance. In: BRAZILIAN CONGRESS OF PLANT PHYSIOLOGY, 16., 2017, São Pedro, SP. Abstract - [S.l.]: Sociedade Brasileira de Fisiologia Vegetal, 2017. p. 109.| Biblioteca(s): Embrapa Agroenergia. |
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| 16. | | FERREIRA, T. M. M.; PAIVA, D. S.; CASARI, R. A. das C. N.; SILVA, V. N. B.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; SOUZA JUNIOR, M. T.; SOUSA, C. A. F. de. Effect of drought on the photochemical apparatus of maize plants as evaluated by parameters derived from chlorophyll fluorescence. In: BRAZILIAN CONGRESS OF PLANT PHYSIOLOGY, 16., 2017, São Pedro, SP. Abstract ... [S.l.]: Sociedade Brasileira de Fisiologia Vegetal, 2017. p. 109-110.| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | CASARI, R. A. C. N.; SILVA, V. N. B.; FERREIRA, T. M. M.; KOBAYASHI, A. K.; DIAS, B. B. A.; SANTOS, T. T.; MOLINARI, H. B. C.; SOUZA JUNIOR, M. T.; SOUSA, C. A. F. de. Use of thermal imaging for discrimination of maize genotypes submitted to water deficit stress. In: LATIN-AMERICAN CONFERENCE ON PLANT PHENOTYPING AND PHENOMICS FOR PLANT BREEDING, 2., 2017, São Carlos, SP. Proceedings... São Carlos: Embrapa Instrumentação, 2017. p. 27.| Biblioteca(s): Embrapa Agroenergia. |
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| 18. | | CASARI, R. A. C. N.; PAIVA, D. S.; SILVA, V. N. B.; FERREIRA, T. M. M.; SOUZA JUNIOR, M. T.; OLIVEIRA, N. G.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; SANTOS, T. T.; GOMIDE, R. L.; MAGALHÃES, P. C.; SOUSA, C. A. F. de. Using thermography to confirm genotypic variation for drought response in maize. International Journal of Molecular Sciences v. 20, n. 9, 2273, May-1, 2019.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Agroenergia; Embrapa Meio-Norte. |
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| 19. | | CASARI, R. A.; PAIVA, D. S.; SILVA, V. N. B.; FERREIRA, T. M. M.; SOUZA JUNIOR, M. T.; OLIVEIRA, N. G.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; SANTOS, T. T.; GOMIDE, R. L.; MAGALHAES, P. C.; SOUSA, C. A. F. de. Using thermography to confirm genotypic variation for drought response in maize. International Journal of Molecular Sciences, v. 20, n. 9, article 2273, 2019.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 19 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Meio-Norte. |
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Data corrente: |
01/11/2022 |
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Data da última atualização: |
01/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FERREIRA, T. M. M.; FERREIRA FILHO, J. A.; LEAO, A. P.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. |
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Afiliação: |
THALITA MASSARO MALHEIROS FERREIRA; JAIRE ALVES FERREIRA FILHO; ANDRE PEREIRA LEAO, CNPAE; CARLOS ANTONIO FERREIRA DE SOUSA, CPAMN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
Structural and functional analysis of stressinducible genes and their promoters selected from young oil palm (Elaeis guineensis) under salt stress. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
BMC Genomics, v. 23, n. 735, p. 1-13, 2022. |
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DOI: |
https://doi.org/10.1186/s12864-022-08926-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Background Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, transcriptional regulatory activity, and opening and closing of stomata. The annotation analysis performed on the promoter sequence revealed 22 distinct types of cis-acting elements, and 14 of them are known to be involved in abiotic stress. Conclusion This study has helped validate the process of an accurate selection of genes responsive to salt stress with a specific and predefined expression profile and their promoter sequence. Its results also can be used in moleculargenetics-assisted breeding programs. In addition, using the identified genes is a window of opportunity for strategies trying to relieve the damages arising from the salt stress in many glycophyte crops with economic importance. MenosBackground Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, trans... Mostrar Tudo |
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Palavras-Chave: |
QRT-PCR; RNA-Seq. |
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Thesaurus NAL: |
Abiotic stress; Salinity; Transcriptomics. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 2147944
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100 1 $aFERREIRA, T. M. M.
245 $aStructural and functional analysis of stressinducible genes and their promoters selected from young oil palm (Elaeis guineensis) under salt stress.$h[electronic resource]
260 $c2022
520 $aBackground Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, transcriptional regulatory activity, and opening and closing of stomata. The annotation analysis performed on the promoter sequence revealed 22 distinct types of cis-acting elements, and 14 of them are known to be involved in abiotic stress. Conclusion This study has helped validate the process of an accurate selection of genes responsive to salt stress with a specific and predefined expression profile and their promoter sequence. Its results also can be used in moleculargenetics-assisted breeding programs. In addition, using the identified genes is a window of opportunity for strategies trying to relieve the damages arising from the salt stress in many glycophyte crops with economic importance.
650 $aAbiotic stress
650 $aSalinity
650 $aTranscriptomics
653 $aQRT-PCR
653 $aRNA-Seq
700 1 $aFERREIRA FILHO, J. A.
700 1 $aLEAO, A. P.
700 1 $aSOUSA, C. A. F. de
700 1 $aSOUZA JUNIOR, M. T.
773 $tBMC Genomics$gv. 23, n. 735, p. 1-13, 2022.
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